實(shí)驗(yàn)室簡(jiǎn)介
實(shí)驗(yàn)室負(fù)責(zé)人駱觀正教授為中山大學(xué)2017年“百人計(jì)劃”引進(jìn)人才，現(xiàn)任生命科學(xué)學(xué)院教授，國家重點(diǎn)實(shí)驗(yàn)室PI，博士生導(dǎo)師。長期以來從事表觀遺傳修飾和基因表達(dá)調(diào)控方面的研究，在生物信息學(xué)和分子生物學(xué)等方面均具有豐富的科研經(jīng)驗(yàn)，系統(tǒng)研究了DNA及RNA甲基化（m6A）修飾對(duì)基因表達(dá)調(diào)控的作用機(jī)制，開發(fā)多個(gè)高通量測(cè)序和計(jì)算方法。詳情可參考：
http://lifesciences.sysu.edu.cn/teachers/professor/258
實(shí)驗(yàn)室致力于發(fā)現(xiàn)和解決關(guān)鍵的生物學(xué)問題，并不拘泥于某一種技術(shù)或某一個(gè)技能，提倡多學(xué)科交叉合作。每一個(gè)加入實(shí)驗(yàn)室的學(xué)生和科研人員都有相對(duì)獨(dú)立的研究方向，彼此有關(guān)聯(lián)但不重疊，每個(gè)人都有充分發(fā)展興趣和培養(yǎng)技能的空間。特別歡迎對(duì)于生命的本質(zhì)有著強(qiáng)烈探索精神的有志青年加入。實(shí)驗(yàn)室將提供一個(gè)相對(duì)寬松的科研文化，歡迎不同背景的同事加入，也會(huì)鼓勵(lì)學(xué)習(xí)和促成合作。目前實(shí)驗(yàn)室經(jīng)過前期建設(shè)，已經(jīng)走上正軌，穩(wěn)定產(chǎn)出結(jié)果。目前作為獨(dú)立實(shí)驗(yàn)室已有一篇Genome Biology和一篇Cell Research發(fā)表，另有多個(gè)課題有望在近期取得突破。
崗位要求
1. 分子生物學(xué)和生物化學(xué)、生物信息學(xué)、細(xì)胞生物學(xué)等相關(guān)專業(yè)博士畢業(yè)生（或?qū)⒂?019年畢業(yè)），符合中山大學(xué)博士后入站基本標(biāo)準(zhǔn)。
2. 品行端正，身心健康，工作責(zé)任心強(qiáng)，善于溝通，具有良好的團(tuán)隊(duì)精神。
3. 協(xié)助培養(yǎng)研究生，協(xié)助指導(dǎo)課題進(jìn)展及撰寫論文。
4. 具有NGS建庫及測(cè)序、單細(xì)胞操作、酶的定向進(jìn)化、化學(xué)修飾檢測(cè)、基因編輯、多組學(xué)聯(lián)合分析等研究經(jīng)驗(yàn)者優(yōu)先。
申請(qǐng)方式
有意者請(qǐng)將詳細(xì)簡(jiǎn)歷（包括學(xué)歷、研究經(jīng)歷、發(fā)表文章等）通過電子郵件發(fā)至：luogzh5@mail.sysu.edu.cn，實(shí)驗(yàn)室報(bào)銷面試差旅費(fèi)。
崗位待遇
1. 基本工資年薪20萬（稅前），實(shí)驗(yàn)室根據(jù)工作成績提供額外獎(jiǎng)勵(lì)。
2. 學(xué)校提供校內(nèi)博士后公寓，享受公費(fèi)醫(yī)療，子女可安排附屬幼兒園入學(xué)。
3. 協(xié)助申請(qǐng)廣東省各類博士后人才項(xiàng)目，工資可有較大幅度上漲。
4. 博士后在站期限為3年，因項(xiàng)目進(jìn)展需要可申請(qǐng)至4年。出站后可繼續(xù)申請(qǐng)專職研究員崗位。優(yōu)秀者可直接應(yīng)聘中山大學(xué)副教授，或廣東省其它高校同等職位。
研究方向
1. NGS測(cè)序及數(shù)據(jù)分析相關(guān)技術(shù)開發(fā)：圍繞DNA和RNA上新型表觀修飾如m6A等熱點(diǎn)問題，開發(fā)新的測(cè)序方法和分析技術(shù)，發(fā)現(xiàn)新的基因表達(dá)調(diào)控規(guī)律。
2. 干細(xì)胞重編程機(jī)制：利用單細(xì)胞測(cè)序等前沿技術(shù)，研究干細(xì)胞去分化、轉(zhuǎn)分化等重編程過程中的表觀遺傳變化規(guī)律，闡明細(xì)胞命運(yùn)決定機(jī)制；探索醫(yī)學(xué)應(yīng)用潛力。
3. 表觀遺傳組和基因轉(zhuǎn)錄調(diào)控：從表觀遺傳角度，探索基因表達(dá)調(diào)控基礎(chǔ)和普遍的機(jī)理。
代表性論文：
2018
N6-methyldeoxyadenosine directs nucleosome positioning inTetrahymena DNA. Genome Biology. 19:200 (2018)
2017
Luo GZ & He C. DNA N6-methyladenine in metazoans: functional epigenetic mark or bystander? Nature Structural & Molecular Biology. 24(6):503-506 (2017)
Roundtree IA, Luo GZ, Zhang Z, Wang X, Zhou T, Cui Y, Sha J, Huang X, Guerrero L, Xie P, He E, Shen B, He C. YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs. Elife. pii: e31311 (2017)
Hsu PJ, Zhu Y, Ma H, Guo Y, Shi X, Liu Y, Qi M, Lu Z, Shi H, Wang J, Cheng Y, Luo G, Dai Q, Liu M, Guo X, Sha J, Shen B, He C. Ythdc2 is an N6-methyladenosine binding protein that regulates mammalian spermatogenesis. Cell Research. 27(9):1115-1127 (2017)
2016
Liu J#, Zhu Y#, Luo GZ#, Wang X#, Yue Y, Wang X, Zong X, Chen K, Yin H, Fu Y, Han D, Wang Y, Chen D, He C. Abundant DNA 6mA methylation during early embryogenesis of zebrafish and pig. Nature Communications.7:13052 (2016)
Luo GZ, Wang F, Weng X, Chen K, Hao Z, Yu M, Deng X, Liu J, He C. Characterization of eukaryotic DNA N6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing. Nature Communications. 7:11301 (2016)
Liu F, Clark W, Luo G, Wang X, Fu Y, Wei J, Wang X, Hao Z, Dai Q, Zheng G, Ma H, Han D, Evans M, Klungland A, Pan T, He C. ALKBH1-Mediated tRNA Demethylation Regulates Translation. Cell. 167(3): 816-828 (2016)
Feng G, Tong M, Xia B, Luo GZ, Wang M, Xie D, Wan H, Zhang Y, Zhou Q, Wang XJ. Ubiquitously expressed genes participate in cell-specific functions via alternative promoter usage. EMBO Reports. 17(9):1304-13 (2016)
2015
Luo GZ, Blanco MA, Greer EL, He C, Shi Y. DNA N6-methyladenine: a new epigenetic mark in eukaryotes? Nature Reviews Molecular Cell Biology. 16(12):705-10 (2015)
Fu Y#, Luo GZ#, Chen K, Deng X, Yu M, Han D, Hao Z, Liu J, Lu X, Doré LC, Weng X, Ji Q, Mets L, He C. N6-Methyldeoxyadenosine Marks Active Transcription Start Sites in Chlamydomonas. Cell. 161(4):879-92 (2015) (# co-first author).
Chen K, Luo GZ, He C. High-Resolution Mapping of N?-Methyladenosine in Transcriptome and Genome Using a Photo-Crosslinking-Assisted Strategy. Methods Enzymol. 560:161-85 (2015)
Shuai L, Wang Y, Dong M, Wang X, Sang L, Wang M, Wan H, Luo G, Gu T, Yuan Y, Feng C, Teng F, Li W, Liu X, Li T, Wang L, Wang XJ, Zhao XY, Zhou Q. Durable pluripotency and haploidy in epiblast stem cells derived from haploid embryonic stem cells in vitro. J Mol Cell Biol. 7(4):326-37 (2015)
Deng X, Chen K, Luo GZ, Weng X, Ji Q, Zhou T, He C. Widespread occurrence of N6-methyladenosine in bacterial mRNA.Nucleic Acids Research. 43(13):6557-67 (2015)
Chen K, Lu Z, Wang X, Fu Y, Luo GZ, Liu N, Han D, Dominissini D, Dai Q, Pan T and He C. High-Resolution N6-Methyladenosine (m6A) Map Using Photo-Crosslinking-Assisted m6A Sequencing. Angew Chem Int Ed Engl. 54(5):1587-90 (2015)
2014
Luo GZ, MacQueen A, Zheng G, Duan H, Dore LC, Lu Z, Liu J, Chen K, Jia G, Bergelson J, He C. Unique features of the m6A methylome in Arabidopsis thaliana. Nature Communications. 5:5630 (2014)
Luo GZ, Yang W, Ma YK, Wang XJ. ISRNA: an integrative online toolkit for short reads from high-throughput sequencing data. Bioinformatics. 30(3):434-6 (2014)
Li W, Li X, Li T, Jiang MG, Wan H, Luo GZ, Feng C, Cui X, Teng F, Yuan Y, Zhou Q, Gu Q, Shuai L, Sha J, Xiao Y, Wang L, Liu Z, Wang XJ, Zhao XY, Zhou Q. Genetic modification and screening in rat using haploid embryonic stem cells. Cell Stem Cell. 14(3):404-14 (2014)
Fu L, Shi Z, Luo G, Tu W, Wang X, Fang Z, Li X. Multiple microRNAs regulate human FOXP2 gene expression by targeting sequences in its 3' untranslated region. Mol Brain. 7:71 (2014)
Before 2013
Luo GZ, Hafner M, Shi Z, Brown M, Feng GH, Tuschl T, Wang XJ, Li X. Genome-wide annotation and analysis of zebra finch microRNA repertoire reveal sex-biased expression. BMC Genomics. 13:727 (2012)
Liu L#, Luo GZ#, Yang W#, Zhao X#, Zheng Q, Lv Z, Li W, Wu HJ, Wang L, Wang XJ, Zhou Q. Activation of the imprinted Dlk1-Dio3 region correlates with pluripotency levels of mouse stem cells. Journal of Biological Chemistry. 285(25):19483-90 (2010) (# co-first author).
Shi Z, Luo G, Fu L, Fang Z, Wang X, Li X. miR-9 and miR-140-5p target FoxP2 and are regulated as a function of the social context of singing behavior in zebra finches. J Neurosci. 33(42):16510-21 (2013)
Wan H, He Z, Dong M, Gu T, Luo GZ, Teng F, Xia B, Li W, Feng C, Li X, Li T, Shuai L, Fu R, Wang L, Wang XJ, Zhao XY, Zhou Q. Parthenogenetic haploid embryonic stem cells produce fertile mice. Cell Research. 23(11):1330-3 (2013)
Zhang Y, Teng F, Luo GZ, Wang M, Tong M, Zhao X, Wang L, Wang XJ, Zhou Q. MicroRNA-323-3p regulates the activity of polycomb repressive complex 2 (PRC2) via targeting the mRNA of embryonic ectoderm development (Eed) gene in mouse embryonic stem cells. J Biol Chem. 288(33):23659-65 (2013)
Li RC, Tao J, Guo YB, Wu HD, Liu RF, Bai Y, Lv ZZ, Luo GZ, Li LL, Wang M, Yang HQ, Gao W, Han QD, Zhang YY, Wang XJ, Xu M, Wang SQ. In Vivo Suppression of MiR-24 Prevents the Transition toward Decompensated Hypertrophy in Aortic-constricted Mice. Circ Res. 112(4):601-5 (2013)
Han J, Chen L, Luo G, Dai B, Wang X, Dai J. Three-dimensional culture may promote cell reprogramming. Organogenesis. 9(2):118-20 (2013)
Liu X, Zhang Y, Tong M, Liu XY, Luo GZ, Xie DF, Ren SF, Bai DH, Wang L, Zhou Q, Wang XJ. Identification of a small molecule 1,4-bis-[4-(3-phenoxy-propoxy)-but-2-ynyl]-piperazine as a novel inhibitor of the transcription factor p53. Acta Pharmacol Sin. 34(6):805-10 (2013)
Li W, Shuai L, Wan H, Dong M, Wang M, Sang L, Feng C, Luo GZ, Li T, Li X, Wang L, Zheng QY, Sheng C, Wu HJ, Liu Z, Liu L, Wang L, Wang XJ, Zhao XY, Zhou Q. Androgenetic haploid embryonic stem cells produce live transgenic mice. Nature.490(7420):407-11 (2012)
Xu M, Wu HD, Li RC, Zhang HB, Wang M, Tao J, Feng XH, Guo YB, Li SF, Lai ST, Zhou P, Li LL, Yang HQ, Luo GZ, Bai Y, Xi JJ, Gao W, Han QD, Zhang YY, Wang XJ, Meng X, Wang SQ. Mir-24 Regulates Junctophilin-2 Expression in Cardiomyocytes. Circ Res. 111(7):837-41 (2012)
Zhang C, Xu Y, Guo S, Zhu J, Huan Q, Liu H, Wang L, Luo G, Wang X, Chong K. Dynamics of brassinosteroid response modulated by negative regulator LIC in rice. PLoS Genet. 8(4):e1002686 (2012)
Du Y, Gao C, Liu Z, Wang L, Liu B, He F, Zhang T, Wang Y, Wang X, Xu M, Luo GZ, Zhu Y, Xu Q, Wang X, Kong W. Upregulation of a disintegrin and metalloproteinase with thrombospondin motifs-7 by miR-29 repression mediates vascular smooth muscle calcification. Arterioscler Thromb Vasc Biol. 32(11):2580-8 (2012)
詳細(xì)論文列表及下載可參考Google scholar:
https://scholar.google.com/citations?user=wqUHZnEAAAAJ&hl=en
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